The equation is C final C initial D where C final is the ending concentration of the diluted solution, C initial is the starting concentration of the original solution and D is the dilution ratio previously determined. 7.Log in Sign up Terms of Use We use cookies to make wikiHow great.Bess Ruff is a Geography PhD student at Florida State University.
She received her MA in Environmental Science and Management from the University of California, Santa Barbara in 2016. She has conducted survey work for marine spatial planning projects in the Caribbean and provided research support as a graduate fellow for the Sustainable Fisheries Group. This article has 18 testimonials from our readers, earning it our reader-approved status. Serial dilutions are used extensively in experimental sciences like biochemistry, microbiology, pharmacology and physics. The liquid that you will be diluting your substance in is very important. Many solutions will be diluted in distilled water, but this is not always the case. If you are diluting bacteria or other cells, you will likely want to dilute in culture media. Determine the number of dilutions you need to do beforehand so you dont waste tubes or diluting liquid. Make A 10 Fold Serial Dilution Serial Dilution IsThe minimum amount needed to perform this serial dilution is 1 mL of undiluted solution. If you only have 1 mL you will not have any remaining undiluted solution. There is now 1mL of the undiluted solution in 9 mL of the dilution liquid. For the second serial dilution, you will take 1 mL of solution from tube 1:10 and add it to the 9 mL of dilution liquid in the tube 1:100. The solution from test tube 1:10 has been diluted 10-fold into test tube 1:100. This process may be repeated as many times as necessary to achieve the desired solution. Make A 10 Fold Serial Dilution Series Of SolutionsIn an experiment involving concentration curves, you can use a serial dilution to create a series of solutions with dilutions of 1, 1:10, 1:100, 1:1,000. The total dilution ratio can be determined by multiplying the dilution factor of each step leading up to the final step. This can be mathematically illustrated with the equation D t D 1 x D 2 x D 3 x x D n where D t is the total dilution factor and D n is the dilution ratio. Plug your dilution factor into the equation: D t 10 x 10 x 10 x 10 10,000. The concentration of your substance is now 10,000 times less than the original undiluted solution. To determine the final concentration of your solution following serial dilution you will need to know your starting concentration. The equation is C final C initial D where C final is the ending concentration of the diluted solution, C initial is the starting concentration of the original solution and D is the dilution ratio previously determined.
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